Mukati
Kune nzira dzakawanda dzekuwana howa mycelium, uye dzakawanda dzadzo dzakasimbiswa kune diki diki mukati memakore ekuedza kunorwadza. Asi kune zvakare nzira dzekugadzira mycelium, iyo isati yakwana uye inoda kumwe kutsvakurudza. Izvi ndizvo zvinoitwa nevanodzidzisa mycologists murabhoritari uye vanorima howa vanorima mycelium nemaoko avo kumba.
Muchisikigo, howa hunonyanya kuberekana nespores, asi hurongwa uhu hunogonawo kuitwa pachishandiswa zvidimbu zvehowa, izvo zvakagara zvakasimbiswa nevarimi vachishandisa mycelium inokura musango sekudyara.
Nzira yekuita mycelium kumba inotsanangurwa zvakadzama pane ino peji.
Vanhu vairima sei mycelium pachavo
Kare, kuitira kukura mamwe marudzi ehowa, semuenzaniso, champignons, vanhu vaitsvaga dunghills uye vakatora mycelium kubva ipapo. Kana mamiriro okunze akanga asina kunaka, uye pakanga pasina mycelium mumarara, saka yakaparidzirwa mune zvakakosha zvekuongorora greenhouses. Nokuda kweizvi, ivhu remupfudze (substrate) rakagadzirirwa uye mycelium yakasimwa ipapo, pasina kuizadza nevhu, kuitira kuti fruiting irege kuitika. Mushure mekumirira kumera kunenge kwakazara kwemycelium musubstrate, varimi vehowa vakatora mycelium ndokuishandisa sekudyara. Mushonga wakadaro wakaomeswa zvishoma unogona kuchengetedzwa kwenguva yakareba.
Munyika Yedu, zvinhu zvekudyara champignon zvakawanikwa nenzira yakafanana kumashure kuma30s. Muzana ramakore rechi XNUMX Nekudaro, pakukura mycelium uchishandisa nzira iyi, goho raive rakashata, mycelium yakakurumidza kupera, uye panguva yekudyara, tupukanana tusingaoneki twaiwanzounzwa, izvo zvaidzivirira kukura kwakajairika kwefungus uye kuderedza fruiting, uye saka masayendisiti akaramba achitsvaga. nzira itsva dzekurima.
Pakupera kwezana remakore reXIX. muFrance, vakakwanisa kugadzirwa kwehuhu husina hutachiona hwema champignon howa hwakarimwa mune yakakosha kudya kunovaka kubva kune spores. Paunenge uchigadzira mycelium mumamiriro akachena, kukwanisa kwe mycelium kwakawedzera zvakanyanya, yakakurumidza kutora midzi, yakakura zvakanyanya muhutano hwekudya uye yakabereka michero kare kare pane kushandisa "wild" hyphae.
Kubva pakati pe20s. Muzana remakore rechi30 marabhoritari akashanda munyika dzakawanda dzinogadzira howa, vaisangoziva nzira yekugadzira mycelium chete, asiwo nzira yekuwana michero yakanakisisa. Muma1932. muUSSR, kunze kwekuwana mycelium pane sterilized compost, zvimwe zvinovaka muviri zvakatsvakwawo nesimba. Muna XNUMX, nzira yekurima mycelium pazviyo zvegorosi yaive patented. Parizvino, varimi vazhinji vehowa pasi rese vari kuita basa rekurima mycelium yezviyo.
Zvakaipa pakukura zviyo mycelium
Sezvo maitiro anoratidza, kuti uwane mycelium, zviyo zvemapira, bhari, oats, gorosi, zviyo, rye nezvimwe zviyo zvinowanzoshandiswa. Paunenge uchiberekesa oyster howa uye zvimwe zvirimwa zvinokura muzvisikwa pahuni, iyo mycelium yekudyara inogadzirwa pazviyo, hura wesunflower, pomace yemazambiringa, sawdust, nezvimwe.
Zvichienderana nemhando yemhando yemuviri inokura iyo mycelium, pane zviyo, substrate, liquid mycelium, nezvimwe.
Aya marudzi ese e mycelium anoratidzwa mumufananidzo:
Liquid mycelium haisati yakajairika, substrate mycelium inoshandiswa zvishoma kazhinji, asi zviyo zvinonyanya kushandiswa. Nekuda kweiyo mycelium yezviyo, nekuda kwekudya kwezviyo, inopa kukurumidza kukura kweiyo mycelium, inoshandiswa muindasitiri howa kukura.
Nekudaro, kugadzirira kweiyo mycelium muindasitiri kana yepamba mamiriro ane zvipingamupinyi. Chokutanga pane zvose, izvi zvinowedzerwa zvinodiwa zvehutano hwezviyo sterilization. Kana iyi nzira isina kubudirira, ipapo mold ichaonekwa, ichidzivirira kukura kweiyo mycelium, izvo zvinozokanganisa huwandu hwechirimwa.
Hupenyu hupfupi hwesherufu yezviyo mycelium (2-3 mwedzi) zvakare chinhu chakakosha chinokanganisa. Mukuwedzera, inofanira kuchengetwa mufiriji pakupisa kwe + 2-5 ° C, sezvo izvi zvichaderedza kukura kwe mycelium. Kana kutonhora kwakakwirira, izvi zvinotungamirira kune chokwadi chokuti kukura kwe mycelium kuchaenderera mberi, semugumisiro wekuti ichakurumidza kudya zvokudya uye kufa.
Nekuonekwa kwe mycelium, hazvibviri kuziva zuva rekugadzirwa kwayo. Chinhu chimwe chete chinogona kukurudzirwa munyaya iyi ndechekuchenjerera paunenge uchitenga parutivi, nokuti mamiriro ekuchengetedza aisagona kuonekwa. Murimi wehowa mutsva anozoona kuti mycelium haina kunaka mwedzi yakawanda gare gare, apo zvichange zvisina maturo kumirira kukohwa.
Izvo zvisingabatsiri zvinogonawo kuverengerwa kune chokwadi chekuti mycelium, yakajaira zviyo, haizo "kuda" kutamira kuhuni.
Ne substrate mycelium, mamiriro acho akasiyana, uye kukanganisa kwayo chete kunoonekwa sekukura zvishoma zvishoma, asi kune mamwe ma pluses: sterility, kukwanisa kuchengeta pamhepo tembiricha kwegore.
Varimi vehowa veAmateur vanowanzofarira substrate mycelium kana vachirima howa pazvimedu zvehuni, sezvo kumhanya kumera hakuna basa pano. Iyi nzira inoenderera mberi kwemwedzi inoverengeka nekuda kwekuwanda kwemuti.
Zvakakosha kuziva kuti mycelium yemhando ipi zvayo inofa kana ikapisa kupfuura 30°C.
Masangano ese ari kuita mukugadzirwa kwe mycelium, uko kwakaringana mamiriro ekurima ayo anogadzirwa. Vamwe vanowana mycelium kumba vaine tarisiro yekuwana mari. Hunhu hwayo hahusi nguva dzose hunosangana nezvinodiwa, asi, mukururama, zvakakosha kuziva kuti dzimwe nguva kune nyanzvi dzakanaka kwazvo.
Howa hunogona kuparadzirwa nemaspores, asi kuparadzira mycelium kunonyanya kufarirwa kune anorima howa, sezvo ichipa mukana uri nani wekubudirira.
Kupfuurirazve, maitiro ekuwana mycelium anoonekwa zvakadzama, sezvo dzimwe nguva zvichingodiwa kuti uzvikure iwe pachako, semuenzaniso, kana nekuda kwechimwe chikonzero mycelium inowanikwa mumamiriro echisikigo (semuenzaniso, zvidimbu zvehuni kana ivhu rakapindwa ne mycelium) hazvina kukwana.
Mapoinzi akakosha ekugadzira mushroom mycelium nemaoko ako ndeaya anotevera. Kutanga, chimedu chisina kuchena chefungal tishu chinobviswa uye chinoendeswa kune inovaka muviri (izvi zvinoitika mumatanho akati wandei, ayo achakurukurwa pazasi). Zvadaro, mienzaniso yakawanda inoumbwa kubva kutsika huru, uye kutarisirwa kunofanira kutorwa kunyanya kudzivirira utachiona hwetsika. Tevere, gadzira nharaunda uye mamiriro anonyanya kuita kuti fruiting yefungus.
Mukuita, tsika inosangana neshanduko dzinotevera: sterile tsika pane agar medium, sterile tsika pazviyo (grain mycelium) uye, pakupedzisira, kubereka muchero pasteurized nutrient medium.
Izwi rekuti "kuora" rinogona kutyisa kune vanotanga, asi zvakakosha kuchengetedza tsika yako yehowa kubva kune akawanda masosi ekusvibiswa aripo munharaunda, zvisinei kuti nharaunda yakachena sei. Zvinonyanya kukosha kuti vadzivise kupinda mutsika yakasimwa, nokuti kana zvisina kudaro pachava ne "kurwa" kwechimiro chekudya, uye chete tsika yehowa inofanira kuishandisa.
Nekumwe kurongeka uye kudzidzira mukuita zvakapusa matekiniki, iyo sterilization maitiro anogona kuitwa nemunhu wese.
Izvi zvinotevera nzira yekugadzira mushroom mycelium agar.
Nzira yekuwana agar ye mycelium kumba
Usati wagadzirira mycelium kumba, unofanira kugadzirira agar nutrient medium. Agar yakagadzirwa kubva kugungwa regungwa, pamwe chete nezvimwe zvekuwedzera, inowanzoshandiswa pakurima kwekutanga uye kunotevera kuparadzaniswa kweiyo fungal tsika.
Nyanzvi dzinowedzera zvakasiyana-siyana zvekudya kune agar, semuenzaniso, zvicherwa, mishonga inorwisa mabhakitiriya, etc. Kukosha kweagar medium kunowanikwawo mukuti microorganisms inokonzera utachiona inogona kuonekwa nyore nyore pamusoro pepakati uye saka zvinokwanisika vabvise pamatanho ekutanga ekurima.
Sezvinoratidzwa nemaitiro, iwe unogona kugadzira mycelium iwe mumhando dzakasiyana dzeagar media. Inonyanya kushandiswa ndeye mbatatisi uye malto-dextrin agar. Iwe unogona kuzvigadzira iwe pachako kana kutenga yakagadzirira-yakagadzirwa musanganiswa wekugadzirwa kwemaindasitiri muchitoro.
Paunenge uchitenga agar muchitoro, iwe uchafanirwa kushandisa imwe mari yakawanda, asi iyo yekuwedzera mari inodzikiswa nekureruka kwekushandisa, uye kana uine mari uye nekushaya nguva yemahara, yakagadzirira-yakagadzirwa musanganiswa ichave sarudzo yakanaka.
Kana iwe wakajaira kuita zvinhu zvose nemaoko ako pachako, saka, maererano nenyanzvi, mbatata agar ye mushroom mycelium kumba inogona kugadzirirwa nenzira mbiri. Nzira mbiri idzi dzinosiyana zvishoma kubva kune imwe neimwe. Mukuwedzera, mushure mekujairana navo, murimi wega wega wehowa anogona kuuya nenzira yake.
Chero zvazvingaitika, kuti ugadzire howa mycelium nenzira iyo tekinoroji inoratidzira, unofanirwa kugadzirira: makapu ekuyeresa, donje bhandiji, foil yealuminium, pressure cooker, test tubes ine screw caps ye autoclaving (inogona kuwanikwa muzvitoro zvemidziyo yekurapa) , chidimbu chiduku chekuzadza test tubes, 2 mabhodhoro ane volume ye 1 l, flasks ane mutsipa wakamanikana.
Zvadaro, iwe uchadzidza kugadzira mbatatisi mycelium agar nenzira yekutanga.
Nzira yekutanga yekugadzirira mbatatisi agar
Inofungidzirwa goho rechinhu i1 litre.
Ingredients: 300 g mbatatisi, 20 g agar (inowanikwa kubva kune yakakodzera marabhu ekurapa, zvitoro zvekudya zvehutano, kana misika yeAsia yechikafu), 10 g dextrose kana imwewo shuga, 2 g yembiriso yedoro (inogona kugoverwa) ).
Nzira yebasa.
Step 1. Usati wagadzira agar ye mycelium ye rough, unoda kubika mbatatisi ne 1 litre yemvura kweawa imwe. Zvadaro bvisa mbatatisi, uchisiya chete muto.
Step 2. Muto, agar, shuga uye mbiriso (kana iwe uchizvishandisa) nyatsosanganisa, semuenzaniso, uchishandisa whisk yekurova, haugone kurova musanganiswa uyu.
Step 3. Dururira musanganiswa unoguma mumabhodhoro kana maflasks nehafu kana zvikamu zvitatu zvehuwandu hwavo.
Vhara mitsipa nekotoni swabs uye kuputira nealuminium foil. Dururira mvura mumudziyo wekumanikidza kuitira kuti dhishi kubva pasi pedhishi riite 150 mm, uye isa gridi yekuisa mabhodhoro kana maflask. Ivhara ndiro nechifukidzo uye sveta machira.
Step 4. Isai steamer pamoto uye mirira kusvikira mhepo yabuda. Mushure mokufefetedza kwemaminetsi mashomanana (zvichienderana nemuenzaniso chaiwo uye maererano nemirayiridzo), vhara valve. Mabhodhoro anobikwa pa121°C (1 atm.) kwemaminitsi gumi nemashanu. Panguva imwecheteyo, zvakakosha kuve nechokwadi kuti kutonhora hakupfuuri chiyero ichi, nokuti munyaya iyi, caramelization yepakati ichaitika, iyo ichaiparadza zvachose.
Step 5. Mushure memaminitsi gumi nemashanu, bvisa chitofu uye usiye ndiro kuti dzitonhore (anenge maminitsi makumi mana). Zvadaro, pasina kutambisa nguva, tora machubhu ebvunzo emahara, bvisa zvivharo uye uise midziyo patripod kana mumagaba akachena, wozoaisa panzvimbo yakacheneswa guruva netsvina.
Step 6. Kana mabhodhoro epakati etsika atonhora, abvise kubva mubiki yekumanikidza uchishandisa tauro kana kicheni mittens. Kusanganisa zvinyoro, bvisa foil uye swabs, uchishandisa funnel, dururira zvirimo mumachubhu ebvunzo nechinenge chikamu chimwe muzvitatu.
Step 7. Vhara machubhu ebvunzo nemakepisi, asi zvishoma kusimba kupfuura kare, zviise mumudziyo wekumanikidza, uchidurura mvura yakawandisa kana zvichidikanwa. Wasvika tembiricha ye121 ° C, siya ndiro pamoto kwemaminetsi makumi matatu, wozoisiya kuti itonhorere zvishoma nezvishoma zvakare kusvikira kudzvanywa kwasvika pane yakajairika.
Step 8. Bvisa machubhu uye pukuta macaps zvakasimba. Gadzirisa machubhu munzvimbo yakarerekera. Nekuda kweizvozvo, pamusoro peiyo agar medium inofanira kunge iri pakona maererano neflask, nekudaro ichigadzira nzvimbo yakawanda sezvinobvira yekuzotevera kukura kwe mycelium (machubhu akadaro dzimwe nguva anonzi "slant agar").
Sezvo iyo yepakati inotonhora, inowedzera uye inowedzera jelly-kufanana mukuenderana uye pakupedzisira inoomesa kusvika pamwero wekuti machubhu anogona kuiswa vertically uye agar medium icharamba iri munzvimbo yayo yekutanga.
Vhidhiyo iyi inotsanangura kugadzirira kwe mycelium agar:
Machubhu anogona kushandiswa pakarepo kana mushure memavhiki kana kunyange mwedzi. Muchiitiko chekupedzisira, dzinofanirwa kuiswa mufiriji, uye usati washandisa, iva nechokwadi chokuti hapana zviratidzo zvehutu kana kusvibiswa kwebhakitiriya pane yepakati.
Chikamu chinotevera chechinyorwa chakatsaurirwa nzira yekuwana mbatatisi mycelium agar kumba neimwe nzira.
Nzira yekugadzira agar ye mycelium kumba nenzira yakasiyana
Inofungidzirwa goho rechinhu i1 litre.
Ingredients:
- 284 g mbatatisi,
- 21,3 g (3/4 oz) agar
- 8 g dextrose (unogona kushandisa tafura yeshuga panzvimbo).
Nzira yebasa.
Step 1. Kuti uite agar ye mycelium nemaoko ako pachako, unoda kushambidza mbatatisi uye ugoicheka kuita zvidimbu zviduku, uchisiya makanda, uye wozoibika mu 0,5 litita emvura kusvikira yakabikwa zvakakwana. Bvisa mbatatisi uye zvimedu zvadzo. Dururira 1 litre yemvura musimbi kana girazi ndiro uye uwedzere dextrose (shuga), decoction uye agar kwairi.
Step 2. Dissolve agar. Kuti uite izvi, isa musanganiswa weagar unoguma mundiro yakafukidzwa nealuminium foil mune yekumanikidza cooker. Pisa mubiki wekumanikidza kusvika 121 ° C (1 atm) uye uende. Mushure memaminitsi makumi maviri, agar ichapera zvachose. Wobva wadzima chitofu woregedza chitofu chitonhorere zvishoma nezvishoma.
Step 3. Uchishandisa mittens yekicheni kana matauro, dururira musanganiswa neagar yakanyungudika mumachubhu ebvunzo (kana mabhodhoro madiki) kusvika pachikamu chetatu chevhoriyamu. Isa machubhu ebvunzo pane rack kana mumagaba. Dururira zvakasara zveagar mubhodhoro, woivhara nekotoni kana padding tampon uye sterize gare gare, pamwe chete nemamwe machubhu ebvunzo.
Makapu ebvunzo machubhu kana zvivharo hazvifanirwe kuvharwa zvakasimba. Muchiitiko ichi, kumanikidzwa kunoenzana panguva ye sterilization. Kana machira ekotoni kana padding tamponi akashandiswa kuvhara, haugone kuita hanya nezvekuenzana kwekumanikidza, zvisinei, nekuwedzera, machubhu ekuyedza anofanirwa kuvharwa nealuminium foil, zvikasadaro iyo condensate yekutonhora yekupisa yekubikira inowira pane inomira.
Step 4. Sterilize agar, iyo machubhu ebvunzo (mabhodhoro) anofanirwa kuiswa mubiki yekumanikidza uye anochengetwa patembiricha ye121 ° C (1 atm.) kwemaminetsi makumi maviri nemashanu, tisingasanganisire nguva inopedzerwa kuwana kudzvanywa kunodiwa. Zvadaro bvisa chitofu uye usiye ndiro kuti dzitonhore zvishoma nezvishoma. Kukurumidza kuderera kwekumanikidzika hakufaniri kubvumirwa, nokuti izvi zvinogona kukonzera agar mumachubhu kuvira, inoputika kunze kuburikidza nemashizha uye zvivharo zvekumisa, izvo zvinogona kutungamirira kuhutachiona.
Step 5. Padanho rekupedzisira, musanganiswa mumachubhu ebvunzo unowana nzvimbo yakarerekera. Kuti uite izvi, pukuta nzvimbo iyo iyo test tubes ichaiswa ne 10% bleach solution ine chlorine. Mumba hamufanirwe kunge mune dhizaini.
Nerubatsiro rwemittens yemukicheni kana tauro kubva muchibikidzo chekumanikidza, tora machubhu ekuyedza anopisa uye woaisa patafura panzvimbo yakarerekera, uchitsamira chigadziko chine magumo kune chimwe chinhu. Izvi zvisati zvaitika, zvinokurudzirwa kusarudza kona chaiyo yekuda kushandisa zvimwe zvinhu zvekunze (mabhawa, murwi wemagazini, nezvimwewo).
Apo agar inotanga kusimba, ichishanduka kuva jelly, vhara mavhavha (mapulagi) mumachubhu ekuedza zvakasimba.
Chengetedza mbatatisi agar mumachubhu ekuedzwa munzvimbo inotonhorera, isina guruva.
Tarisa vhidhiyo yekugadzira agar ye mycelium nemaoko ako pachako:
Chikamu chekupedzisira chechinyorwa chakatsaurirwa nzira yekukura zvakanaka mushroom mycelium.
Nzira yekubika mushroom mycelium kumba
Usati wakura mycelium kumba, gadzirira: scalpel (banga rakapinza rine bango rakatetepa), mwenje wemweya (tochi yepropane ine canister, mwenje kana machisi), magaba esimbi kana machira emachubhu ebvunzo ane slant agar uye bvunzo dzakagadzirirwa. machubhu, scalpel chibatiso kana banga, microporous bhandeji (standard bhandeji rakanaka), pfapfaidza bhodhoro rine musanganiswa wechikamu 1 chebleach neklorini uye 9 zvikamu zvemvura (optional), nyowani yakachena howa inobereka muviri (kana iwe uri wekutanga. zvakanakisisa kushandisa oyster howa).
Nzira yebasa.
Step 1. Usati wakura mycelium, unofanirwa kugadzirira nzvimbo yakagadzikana (tafura, counter) nekuishambidza nemvura inodziya ine sipo uye kuipukuta yakaoma. Kuti upe humwe hutachiona hwehutachiona, bata nzvimbo neaerosol ine 10% bleach solution, pukuta zvakakwana nemucheka wakachena kana pepa. Vhara mahwindo kudzivirira kutenderera kwemhepo zvakanyanya sezvinobvira. Zviri nani kuita basa mumaawa emangwanani, kana paine guruva shoma mumhepo.
Step 2. Kuti ukure mycelium kumba, unofanirwa kuronga nzvimbo yekushanda: gadzira maturusi uye zvinhu mukati mekusvika uye mune yakanakira kurongeka, yakagadzirira kushanda.
Tora machubhu eagar woaisa mumagaba esimbi kana pamarekeni. Batidza chiedza uye unyatso sira blade yebanga (scalpel) mumoto, uiise pachigadziko, somuenzaniso, yakagadzirwa newaya. Chigadziko chinodiwa kuitira kuti badza rebanga rigare riri pedyo nemoto asi mudziyo usiri kushandiswa.
Step 3. Tora howa yakachena yakachena. Kunyangwe nzvimbo yaro yekunze inogona kunge iine hutachiona hwakawanda uye molds, kazhinji hapana zvipenyu mukati memukati tishu zvinogona kukonzera hutachiona, hongu, kana pasina mvura yakawandisa mufungus.
Hazvibviri kuputsa chikamu chefungus, nokuti blade inokonzera mukati mefungus nebhakitiriya kubva kunze. Isai mushroom patafura ine tsvina yakasviba (yakachena haifaniri kusvika pakusangana netafura).
Chinhu chakakosha ndechekuti iwe unofanirwa kuumba nzvimbo yakachena yakavhurika, uye wozotora chidimbu chidiki chehowa tishu kubva mairi, iyo inoiswa muyedzo chubhu.
Step 4. Kuti ukure mycelium iwe pachako, ronga zvishandiso uye zvinhu nenzira yekuti test tube inovhurwa zvishoma sezvinobvira isati yazadza nehowa tishu. Kuti uderedze mukana wehutachiona, test tube (kana chivharo, chivharo) haifaniri kuiswa panzvimbo yebasa, iyo yakaoma zvikuru, saka zvine musoro kudzidzira nechubhu yekuedza isina chinhu mberi.
Step 5. Kuwedzera kutevedzana kunonyanya kutsanangurwa kana ruoko rworudyi kana rworuboshwe ruchiita basa iri, zviito zvemunhu ane ruoko rworudyi zvinotsanangurwa pasi apa.
Chigunwe chekuruboshwe chiri pasi, asi mamwe acho akachinjika. Isa chubhu yekuedza pakati pepakati neminwe yemhete. Muchiitiko ichi, chigunwe chemhete chiri pamusoro, chigunwe chepakati chiri pasi peflask, uye cork (chivharo) inotungamirirwa kubva paruoko. Izvo hazvifanirwe kurereka test chubhu, chete nzvimbo yakachinjika inodiwa pano, zvikasadaro zvimedu zvinobhururuka mumhepo zvichave nemukana wakakura wekupinda mumutsipa wemudziyo. Iko kutaridzika kwechubhu ndeyekuti iyo bevelled surface ye agar inotungamirirwa kumusoro. Iri pairi kuti mushroom tissue ichasimwa.
Step 6. Nyatsobvisa chivharo (chivharo) kubva pachubhu yekuedza uye tora iyo yekupedzisira nenzira yakaratidzwa.
Uchishandisa chigunwe chemahara uye chigunwe cheruoko rweruboshwe, tora chidimbu chehowa ine nzvimbo yakachena. Noruoko rwako rworudyi, kurumidza kutora scalpel nenzira yakadai sepenzura kana peni. Uchishandisa muromo weblade, nyatso bvisa chidimbu chidiki chehowa hwematatu kubva kune yakachena mushroom tishu uye, nekukurumidza, woiisa mufurasiki pamucheto wemutsipa, kana zvichidikanwa, uchizunza kubva kumucheto we scalpel nekugogodza. kufamba. Isa scalpel kumashure uye nekukurumidza kuvhara chubhu nechivharo.
Step 7. Bata chubhu zvishoma paruoko rwako kufambisa chidimbu chefungus pane agar pamusoro. Isa chubhu mune rimwe tin rakagadzirirwa kuchengetedza machubhu anoculated.
Kana kurudziro dzikateverwa chaizvo, pane mukana wakanaka wekuti yakasimwa howa tsika yaive yakachena.
Kutevedzana kwakafanana kwezviito kunoitwa nemamwe maflask uye mushroom zvinhu. Zvakakosha kugadzirira machubhu akawanda ekuedza kubva kune imwe mushroom, nokuti pasinei nokuti basa rinoitwa sei nekuchenjerera uye nekuchena, zvirwere zvinowanzoitika.
Mushure mekunge zvinhu zvehowa zvapinzwa muchubhu yekuyedza (iyi nzira inonzi inoculation), scalpel inofanira kuvharirwa zvakare pamoto.
Kana wapedza nemachubhu ebvunzo, unofanirwa kuvhara chivharo zvakasimba sezvinobvira uye kuputira nzvimbo yacho ne microporous tepi, iyo isingazodzivisi fungus kubva "kufema" uye panguva imwechete haibvumiri mabhakitiriya kupinda mu test tube kuburikidza. mutsipa.
Zvinokurudzirwa kuisa zvimiti pafuraski yega yega kana kuita zvinyorwa zvine mucherechedzo unoratidza zuva uye ruzivo nezve zviri mukati.
Yakagadzirira bvunzo machubhu anochengetwa munzvimbo ine rima uye inotonhorera pane yakanyanya tembiricha ye13-21 °C. Mushure meimwe nguva (mazuva akati wandei kana vhiki), huturu hwehowa huchazara ne fluff, iyo inoratidza kutanga kwekukura kwe mycelium. Mushure memamwe mavhiki mashoma, mycelium ichafashamira nzvimbo yese yeagar.
Muhupo hwehutu, hunoonekwa nyore nyore negirinhi kana dema spores, kana kusvibiswa kwebhakitiriya (sekutonga, inoratidzika sechinhu chine ruvara chinopenya), zviri mukati me test tube zvinofanira kuraswa pakarepo uye kushambidzwa nemvura inopisa ine sipo pamwe chete. nekoki. Kana zvichibvira, machubhu ebvunzo ane utachiona anovharirwa mune imwe kamuri umo musina tsika dzine hutano.
Tsanangudzo yekukura kwe mycelium inotsanangurwa muvhidhiyo iyi:
